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Title   난소암 세포주에서 Protein Kinase C 활성조절제들에 의한 Telomerase 활성도의 변화 ( Changes of Telomerase Activity by Protein Kinase C Modulators in Human Ovarian Cancer Cell Lines )
Publicationinfo   2000 Jan; 032(04): 724-734.
Key_word   Ovary neoplasm, Telomerase, Protein kinase C modulator
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Abstract   Purpose: This study was designed to find out whether protein kinase C (PKC) may affect telomerase activity in human ovarian cancers. Materials and Methods: To determine whether PKC modulators influence PKC activities, NIH: OVCAR-3 and CUMO-2, cells were treated with PKC inhibitors, GO6976 and bisindolylmaleimide I, and PKC activator, 12-0-tetradecanoyl phorbol 13-acetate (TPA). Telomerase acti- vity was determined by telomeric repeat amplification protocol (TRAP). Analysis of the expres- sion of each telomerase subunits, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT), was performed by RT-PCR. We also examined the alternative splicing of hTERT. Results: GO6976 and bisindolylmaleimide I inhibited PKC activity. Telomerase activities appeared to be affected in a time-dependent manner by these two PKC inhibitors. PKC activities were increased in parallel with telomerase activity by TPA at the low dose (10 nM), but their activities were down-regulated at the high dose (1 pM). RT-PCR demonstrated the presence of hTR and hTERT mRNA before and after the treatment of PKC modulators, respectively, and showed the presence of one alternatively spliced transcript and full-length hTERT transcripts. Conclusion: These results showed that telomerase activity was affected by PKC and suggested PKC modulation may serve as an useful tool in the regulation of telomerase activity.
저 자   허수영(Soo Young Hur),이준모(Joon Mo Lee),남궁성은(Sung Eun Namkoong),김진우(Jin Woo Kim)